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Haematoxylin-Eosin staining, Masson Trichrome staining and immunohistochemical staining with endothelial nitric oxide synthase (eNOS) (Novus Biologicals, NB300-500, UK), neuronal nitric oxide synthase (nNOS) (BIOSS, BS-0156R, USA), alpha-smooth muscle actin (α-SMA) (Novus Biologicals, NBP1-30894, UK) primary antibodies were performed

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As leading symptoms, increased intraocular pressure and mydriasis, as well as degeneration of retinal ganglion cells, optic nerve head excavation and reduction in optic nerve thickness, generalized severe irregularity of retinal vessels, faint presentation of choroidal vessels and severe optic nerve disc atrophy were all counteracted