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Capsules:30
#8231 Buffer • AmBic buffer: 100 mmol/L ammonia bicarbonate in HPLC water • Cell lysis buffer: 10 mol/L urea in 100 mmol/L AmBic buffer with protease (one tablet in 40 mL) and phosphatase inhibitors (100× ) • Precipitation buffer: 50% ethanol + 49.9% acetone + 0.1% acetic acid • Buffer A: 2% acetonitrile + 97.9% HPLC water + 0.01% formic acid • Buffer B: 80% acetonitrile + 19.9% HPLC water + 0.01% formic acid • Binding/Washing buffer: 80% acetonitrile + 19.9% HPLC water + 0.01% trifluoroacetic acid • Elution buffer: 50% acetonitrile + 45% HPLC water + 5% ammonia solution Equipment • Orbitrap Fusion Lumos Tribrid mass spectrometer, Thermo Scientific • EASY-nanoLC 1200 systems, Thermo Scientific • VialTweeter device, Hielscher-Ultrasound Technology • Nanodrop, Thermo Scientific • SPD111V, Thermo Scientific • VORTEMP 56, Labnet • SUPELCO vacuum manifold system • ThermoMixer, F2.0 Software • Spectronaut v16, Biognosys, Inc
