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While their mechanisms of action are similar, topical acetyl hexapeptide-8 does demonstrate a lower efficacy than injected Botox, but — as researchers note — this is to be expected.22 Similar results were found in a small 2013 study, with the “total anti-wrinkle efficacy” of the Argireline treatment group being 48.9% compared to 0% in the placebo group.23 However, a more recent 2023 study investigating the effects of serum containing Argireline and hyaluronic acid found the results to be “not statistically significant.”24 This means that more research is needed before claims that Argireline is a true alternative to Botox can be confirmed or denied

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In this study, we used LOXL2 as the representative protein of the LOX family to develop and validate this new assay