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Smoking as well as hypertension, dyslipidemia, diabetes, obesity, aging, lack of exercise, excessive salt intake, and menopause are known to induce endothelial dysfunction [7]

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Since each column has its unique pH range of analysis, it is crucial to note that pH must be maintained prior to analysis so as to avoid any degradation or change of the stationary phase and, subsequently, the compromise of the column (Wichitnithad et al., 2009)