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For first-strand cDNA synthesis, 500 ng of total RNA were used with an equimolar mixture of four oligo(dT) primers (CDSIII-NotIA/C/G/T, Supplementary Table 7) and RevertAid H Minus Reverse Transcriptase (Thermo Scientific)

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TR-FRET Titrations of compounds to induce the DCAF11–DDX18 complex were carried out by mixing biotinylated DCAF11 and GFP–DDX18 at the concentration described in the figure legend, and 2 nM terbium-coupled streptavidin (prepared in-house) in an assay buffer containing 50 mM HEPES pH 8.0, 200 mM NaCl, 1 mM TCEP, 0.05% Tween-20, and 1 mM TCEP