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High-frequency sleep fragmentation in obstructive sleep apnea has been shown to induce outcomes similar to chronic sleep deprivation, including reduced antioxidant enzyme activity (e.g., superoxide dismutase, glutathione peroxidase), aberrant mitochondrial morphology, and diminished ATP production, all of which impair neuronal metabolism ( 5 Sleep and mitochondrial health affect peripheral systems including the immune system and gut 5.1 Mitochondrial decline, inflammaging, and sleep The concept of “inflammaging,” first introduced by Claudio Franceschi in 2000, refers to the chronic, low-grade systemic inflammation that characterizes biological aging (Franceschi et al., 2000

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The resulting supernatants were diluted in a 10 times volume, and the dilutions were used in the assay